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    • Analytical biochemistry Journal

    Papers
    Increased Uniformity in the Response of the Coomassie Blue G-Protein Assay to Different Proteins
    Saadet Demirtas Inci, Murat Tulmac,Cagatay Tunca, Tolgahan Efe,Hakan Gullu
    Analytical Biochemistry（1990）SCI 4区
    In this report, we present a patient with ventricular septal defect (VSD) that was detected at follow-up one month after transcatheter aortic valve implantation (TAVI) and successfully closed percutaneously. Before the procedure, a 29 mm Portico self-expanding aortic valve prosth...
    Cited171Views5
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    Recombinant Technology As an Alternative to Chemical Peptide Synthesis: Expression and Characterization of HIV-1 Rev Recombinant Peptides
    P LEPAGE,C HECKEL,S HUMBERT, S STAHL,G RAUTMANN
    Analytical Biochemistry（1993）SCI 4区
    Recombinant DNA technology has been widely used for the production of proteins in recent years. In this paper, we describe the expression and the purification of two specific peptides corresponding to parts of the human immunodeficiency virus Rev protein. The strategy of this met...
    Cited25Views4
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    A Method for Controlled Coupling of Amino Compounds to Enzymes: A Homogeneous Enzyme Immunoassay for Gentamicin
    P SINGH,DK LEUNG,GL ROWLEY,C GAGNE,EF ULLMAN
    Analytical Biochemistry（1980）SCI 4区
    A new method for coupling of amino compounds to enzymes is described. An aminoglycoside, gentamicin, is functionalized with a nucleophilic sulfhydryl group on its deoxystreptamine ring and an enzyme, glucose 6-phosphate dehydrogenase (G6PDH), is modified with electrophilic bromoa...
    Cited8Views3
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    Analysis of Variance of Parameter Estimates: F Tests and T Tests
    LA JOHNSON
    Analytical Biochemistry（1992）SCI 4区
    The problem of comparing and pooling experimentally independent estimates of a parameter such as a Michaelis constant (K) has been treated as a simple analysis of variance of “within” and “between” set deviations from the fitted variable (v). As applied to assessing the reproduci...
    Cited6Views2
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    Portable Quantification of Silver Ion by Using Personal Glucose Meter (PGM) and Magnetite Cross-Linked Invertase Aggregates (MCLIA)
    Ilker Polatoglu,Aysenur Yardim
    Analytical Biochemistry（2021）SCI 4区
    Heavy metal detection is critical due to its harmful effects on human health and the ecosystem. Enzyme-based platforms attract attention for heavy metal detection such as silver, a toxic metal, due to being small, portable, and requiring only essential equipment compared with the...
    Cited3Views5
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    Determination of Endogenous Concentrations of N1-methylnicotinamide in Human Plasma and Urine by High-Performance Liquid Chromatography
    A SOMOGYI,D SIEBERT,F BOCHNER
    Analytical Biochemistry（1990）SCI 4区
    A high-performance liquid chromatographic assay for the determination of endogenous plasma and urine concentrations of N1-methylnicotinamide was developed. N1-Methylnicotinamide and N1-ethylnicotinamide (internal standard) are reacted with acetophenone in a strong base at 0 degre...
    Cited21Views4
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    Electrocatalytic Artificial Carbonylation Assay for Observation of Human Serum Albumin Inter-Individual Properties
    Jan Vacek,Marika Svrckova,Martina Zatloukalova,David Novak,Jitka Proskova,Katerina Langova,Dana Galuszkova,Jitka Ulrichova
    Analytical Biochemistry（2018）SCI 4区
    Human serum albumin (HSA) is a multifunctional protein with ligand binding, transporting and buffering properties. Posttranslational modifications and ligand binding processes are closely related to albumin final functional status. In the last few decades, HSA has been characteri...
    Cited8Views25
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    Exponential ATP Amplification Through Simultaneous Regeneration from AMP and Pyrophosphate for Luminescence Detection of Bacteria
    Hui-Ju Lee,Min-Rong Ho,Chih-Sian Tseng,Ching-Yi Hsu,Meng-Shun Huang,Hwei-Ling Peng,Hwan-You Chang
    Analytical Biochemistry（2011）SCI 4区
    Bacteria monitoring is essential for many industrial manufacturing processes, particularly those involving in food, biopharmaceuticals, and semiconductor production. Firefly luciferase ATP luminescence assay is a rapid and simple bacteria detection method. However, the detection ...
    Cited11Views2
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    Membrane Proteins Lose Activity on Freezing Because of Adsorption: Implications for Activity and Protein Assays
    SV PANDE,MSR MURTHY
    Analytical Biochemistry（1994）SCI 4区
    Meaningful estimates of diacylglycerol acyltransferase (EC 2.3.1.20) activity in different tissue samples require effective, unbiased methods of sample storage. Samples of the pars costalis diaphragmatis muscle (skirt muscle of the diaphragm) were obtained from 18- to 20-month-ol...
    Cited4Views0
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    Quantum DFT Methods to Explore the Interaction of 1-Adamantylamine with Pristine, and P, As, Al, and Ga Doped BN Nanotubes
    Ebrahim Nemati‐Kande, Amir Pourasadi,Fatemeh Aghababaei,Samaneh Baranipour, Ata Mehdizadeh,Jaber Jahanbin Sardroodi
    Analytical Biochemistry（2022）SCI 4区
    Nanostructures, nowadays, found growing applications in different scientific and industrial areas. Nano-coins, nanosheets, and nanotubes are used in medical applications as sensors or drug delivery substances. The aim of this study is to explore the adsorption of 1-Adamantylamine...
    Cited17Views7
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    Fluorescent Copper Nanoclusters As a Nano-Dye for DNA Methyltransferase Activity Analysis and Inhibitor Screening
    Dengpeng Gao, Hongyue Zhang,Yafei Xu, Yun Liu, Huiying Xu, Jianguo Cui
    Analytical Biochemistry（2018）SCI 4区
    Fluorescent copper nanoslusters (CuNCs) as a new class of fluorophores have attracted more and more attention due to their ease of synthesis, excellent optical properties, and low cost. In this study, a novel label-free fluorescent method was developed for the detection of DNA me...
    Cited7Views2
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    A High-Throughput System to Verify Candidate Interactors from Yeast Two-Hybrid Screening Using Rolling Circle Amplification
    XD Ding,G Cory,WY Song
    Analytical Biochemistry（2004）SCI 4区
    Cys2/His2-type zinc finger proteins, which contain the EAR transcriptional repressor domain, are thought to play a key role in regulating the defense response of plants to biotic and abiotic stress conditions. Although constitutive expression of several of these proteins was show...
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    Interactions of Myristoylated Alanine-Rich C Kinase Substrate (Marcks)-Related Protein with a Novel Solid-Supported Lipid Membrane System (TRANSIL)
    AAP Schmitz,E Schleiff,C Rohring,A Loidl-Stahlhofen,G Vergeres
    Analytical Biochemistry（1999）SCI 4区
    The determination of partition coefficients is crucial for the biochemical analysis of membrane-based processes, but requires tedious procedures. We have facilitated this analysis using a silica gel coated with a single phospholipid bilayer (TRANSIL) as the membranous phase. We d...
    Cited22Views6
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    Differences in Responsivity of Original Cupric Reducing Antioxidant Capacity and Cupric–bathocuproine Sulfonate Assays to Antioxidant Compounds
    S. Esin Celik,Mustafa Ozyurek,Kubilay Guclu,Resat Apak
    Analytical Biochemistry（2012）SCI 4区
    The cupric reducing antioxidant capacity (CUPRAC) method using bathocuproine sulfonate (BCS) as cuprous-chelating ligand claims to be an efficient substitute for the conventional CUPRAC assay using neocuproine; however, differences in their physical chemistry give rise to signifi...
    Cited13Views3
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    Maximizing Hydrophobic Peptide Recovery in Proteomics and Antibody Development Using a Mass Spectrometry Compatible Surfactant
    Song Nie,Reid O'Brien Johnson,Yuliya Livson,Tyler Greer,Xiaojing Zheng,Ning Li
    Analytical Biochemistry（2022）SCI 4区
    Peptide loss due to surface absorption can happen at any step in a protein analysis workflow and is sometimes especially deleterious for hydrophobic peptides. In this study, we found the LC-MS compatible surfactant, n-Dodecyl-β-D-maltoside (DDM), can maximize hydrophobic peptide ...
    Cited6Views17
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    Continuous Fluorescence-Based Measurement of Redox-Driven Sodium Ion Translocation
    Valentin Muras,Bjoern Claussen,Manikandan Karuppasamy,Christiane Schaffitzel,Julia Steuber
    Analytical Biochemistry（2014）SCI 4区
    Investigation of the mechanism of sodium ion pumping enzymes requires methods to follow the translocation of sodium ions by the purified and reconstituted proteins in vitro. Here, we describe a protocol that allows following the accumulation of Na(+) in proteoliposomes by the Na(...
    Cited2Views24
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