In-Source Decay and Pseudo-MS3 of Peptide and Protein Ions Using Liquid AP-MALDI
Journal of the American Society for Mass Spectrometry（2016）SCI 3区SCI 2区
Fondazione Pisana per la Scienza ONLUS
Abstract
Atmospheric pressure MALDI on a Q-Exactive instrument was optimized for in-source decay and pseudo-MS3. The dependence of AP-MALDI ISD on the MALDI liquid matrix was investigated for peptides and proteins. The liquid matrices enabled long-life ISD signal, and exhibited high fragment ion yield and signal stability. Extensive a-, b-, c-, y-, and z-type fragment series were observed depending on the matrix used but were most extensive with 2,5-DHB. Complete sequence coverage of small peptide and intact protein-terminus sequence tags were obtained and confirmed using HCD as a pseudo-MS3 method. Graphical Abstract ᅟ.
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Key words
MALDI,In-source decay,MS/MS,Pseudo MS3,T3 sequencing
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