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Structural Identification of Compounds for Use in the Detection of Juice-to-juice Debasing Between Apple and Pear Juices

Food Chemistry(2018)SCI 1区

Univ Saskatchewan

Cited 22|Views12
Abstract
The ability to detect the undeclared addition of a juice of lesser economic value to one of higher value (juice-tojuice debasing) is a particular concern between apple and pear juices due to similarities in their major carbohydrate/polyol profiles. Fingerprint compounds for the detection of this type of adulteration were identified in both commercial apple and pear juices by HPLC-PDA, were isolated chromatographically, and structurally identified by LC-MS/MS. The apple juice fingerprint was identified as 4-O-p-coumarylquinic acid and two pear compounds as isorhamnetin-3-O-rutinoside and abscisic acid. Additionally, the HPLC-PDA profile of pear juices in combination with pear fingerprint compounds including arbutin could be used to identify samples originating from China versus those from other geographical locations.
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Authenticity,Phenolics,Mass spectrometry,Apple and pear juice
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要点】:本文通过高效液相色谱-二极管阵列检测器(HPLC-PDA)和液相色谱-串联质谱(LC-MS/MS)技术,成功鉴定出用于检测苹果汁与梨汁之间低成本果汁非法添加的指纹化合物,并能够区分来自中国和其他地区的梨汁样本。

方法】:研究者采用HPLC-PDA技术分析商业苹果汁和梨汁,分离出色谱指纹化合物,并通过LC-MS/MS技术进行结构鉴定。

实验】:实验中使用了商业苹果汁和梨汁作为样本,通过HPLC-PDA和LC-MS/MS分析,鉴定出苹果汁指纹化合物为4-O-p-香豆酰奎宁酸,梨汁中则为异鼠李素-3-O-芸香糖苷和脱落酸。同时,结合梨汁的HPLC-PDA谱图和指纹化合物(包括熊果苷),能够区分出产自中国与其他地区的梨汁样本。