Reproducibility of CRISPR-Cas9 Methods for Generation of Conditional Mouse Alleles: a Multi-Center Evaluation.

Gurumurthy Channabasavaiah B., The Australian National University,Quadros Rolen M., Adams John,Alcaide Pilar,Ayabe Shinya,Ballard Johnathan,Batra Surinder K.,Beauchamp Marie-Claude, Becker Kathleen A., Bernas Guillaume,Brough David,Carrillo-Salinas Francisco,Chan Wesley,Chen Hanying,Dawson Ruby,DeMambro Victoria, Ghent University,Dibb Katharine,Eudy James D.,Gan Lin, Gao Jing,Gonzales Amy,Guntur Anyonya,Guo Huiping,Harms Donald W.,Harrington Anne,Hentges Kathryn E.,Humphreys Neil, Imai Shiho,Ishii Hideshi, Iwama Mizuho,Jonasch Eric, Karolak Michelle,Keavney Bernard,Khin Nay-Chi,Konno Masamitsu,Kotani Yuko,Kunihiro Yayoi,Lakshmanan Imayavaramban,Larochelle Catherine,Lawrence Catherine B., Li Lin,Lindner Volkhard,Liu Xian-De,Lopez-Castejon Gloria,Loudon Andrew,Lowe Jenna, Jerome-Majeweska Loydie,Matsusaka Taiji,Miura Hiromi,Miyasaka Yoshiki,Morpurgo Benjamin,Motyl Katherine,Nabeshima Yo-ichi,Nakade Koji,Nakashiba Toshiaki,Nakashima Kenichi,Obata Yuichi,Ogiwara Sanae, Ouellet Mariette,Oxburgh Leif,Piltz Sandra,Pinz Ilka,Ponnusamy Moorthy P.,Ray David, Redder Ronald J.,Rosen Clifford J., Ross Nikki, Ruhe Mark T.,Ryzhova Larisa,Salvador Ane M.,Alam Sabrina Shameen,Sedlacek Radislav,Sharma Karan,Smith Chad,Starrs Lora,Sugiyama Fumihiro,Takahashi Satoru,Tanaka Tomohiro,Trafford Andrew,Uno Yoshihiro,Willis Brandon J.,Wright Christian S.,Yamauchi Yuko,Yi Xin,Yoshimi Kazuto,Zhang Xuesong,Zhang Yu,Ohtsuka Masato,Das Satyabrata,Garry Daniel J.,Thomas Paul,Parker-Thornburg Jan,Adamson Antony D.,Yoshiki Atsushi,Schmouth Jean-Francois,Golovko Andrei,Thompson William R., University of California, Davis,Wood Joshua A.,Cowan Mitra,Mashimo Tomoji,Mizuno Seiya,Zhu Hao,Kasparek Petr,Liaw Lucy,Miano Joseph M.,Burgio Gaetan

Genome Biology（2021）

Cited 51|Views56

Key words

CRISPR-Cas9,Mouse,Transgenesis,Homology-directed repair,Conditional knockout mouse,Floxed allele,Oligonucleotide,Long single-stranded DNA,Machine learning,Reproducibility

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