Development of an RNA Strand-Specific Hybridization Assay to Differentiate Replicating Versus Nonreplicating Influenza A Viruses.
Journal of clinical microbiology(2020)
Ctr Dis Control & Prevent
Abstract
Replication of influenza A virus (IAV) from negative-sense viral RNA (vRNA) requires the generation of positive-sense RNA (+RNA). Most molecular assays, such as conventional real-time reverse transcriptase PCR (rRT-PCR), detect total RNA in a sample without differentiating vRNA from +RNA. These assays are not designed to distinguish IAV infection versus exposure of an individual to an environment enriched with IAVs but wherein no viral replication occurs. We therefore developed a strand-specific hybridization (SSH) assay that differentiates between vRNA and +RNA and quantifies relative levels of each RNA species.
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Key words
RNA species,single-strand hybridization (SSH) assay,replication,influenza A virus (IAV) infection
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