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濒危水生植物水角的离体培养技术

Jiangsu Agricultural Sciences(2019)

Cited 1|Views24
Abstract
为建立有效的水角离体培养技术,以水角的种子和茎段诱导出不定芽,采用单因素方差分析和Duncan's多重比较法比较不同处理间的差异性,研究糖类、pH值、细胞分裂素和生长素对水角生长状况的影响.结果表明,以种子为外植体,0.1%HgCl2消毒8 min进而诱导,出现污染率30%,褐化率20%,萌芽率68%,芽点数5.07个;适宜的增殖培养条件为蔗糖30.00 g/L,pH值为6和6-BA 1.00 mg/L,增殖系数均为比较组中的最优值;NAA 0.20 mg/L较适合水角生根壮苗,根数达6.42条,最长根长9.33 cm,株高10.37 cm,茎粗0.31 cm.水角的离体培养技术探索将有效加快种苗快繁,为濒危水生植物水角的种质保存提供技术基础.
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