一种大量提取猕猴桃不同组织高质量总RNA的方法
Acta Agriculturae Jiangxi（2020）
Abstract
在CTAB法的基础上,经过多方面改进,提取了"红阳"猕猴桃7种组织(果实、雄花、雌花、叶片、种子、皮、根)的总RNA.利用琼脂糖凝胶电泳和超微量核酸蛋白测定仪检测了该方法和试剂盒法提取的RNA的完整性、纯度和浓度.结果 显示:采用这两种方法提取到的猕猴桃不同组织的总RNA,能检测到18S和28S两条清晰完整的条带,A260/A280值在1.8～2.0间,A260/A230值大于2.0.进一步的RT-PCR验证结果表明采用这两种方法提取到的RNA质量较高,均达到了分子生物学实验的要求.因此,本文改良的CTAB法适用于猕猴桃各组织高质量总RNA的大量提取.
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