四君子汤对人乳腺癌MDA-MB-231细胞生长及PD-L1表达的影响
Traditional Chinese Medicinal Research（2019）
Abstract
目的:观察四君子汤对三阴性乳腺癌(triple negative breast cancer,TNBC)细胞MDA-MB-231增殖、凋亡及程序性死亡分子配体-1 (programmed cell death ligand 1,PD-L1)表达的影响.方法:以四君子汤煎煮浓缩液处理人TNBC细胞MDA-MB-231,分别在24,48,72 h,采用细胞增殖/毒性实验的方法检测高(2 g/L)、中(1 g/L)、低剂量(0.1 g/L)四君子汤在不同时间点对MDA-MB-231细胞增殖的影响;采用流式细胞术检测高、中、低剂量四君子汤在48 h时对MDA-MB-231细胞凋亡的影响;采用Western blot技术检测加药48 h时四君子汤高、中、低剂量组MDA-MB-231细胞中PD-L1的表达情况.结果:与空白对照组对比,3种剂量四君子汤处理不同时间对细胞增殖影响无显著性差异;四君子汤处理72 h后,高、中、低剂量组抑制率分别为10.21％、2.97％和0.12％,其中高剂量组抑制率显著低于紫杉醇组(49.88％),差别有统计学意义(P＜0.05).四君子汤高、中、低剂量组48 h,凋亡率分别是24.25％、16.90％和15.37％,与空白对照组对比,四君子汤高剂量组凋亡率显著增高(P＜0.05).与空白对照组对比,四君子汤高剂量组处理48h后,PD-L1表达显著降低(P＜0.05).结论:四君子汤对TNBC细胞MDA-MB-231的增殖无明显影响,高剂量四君子汤能够诱导其凋亡,并降低PD-L1的表达.
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