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    • 一株新疆罕见野生大型真菌的形态及分子生物学鉴定

    袁江玲,雒婉霞,徐晓辉,徐佳丽,冯玉明

    Endemic Diseases Bulletin（china)（2020）

    Cited 1|Views33
    Abstract
    目的 对一株采自新疆境内的罕见大型野生真菌进行形态学及分子生物学鉴定.方法 采用传统形态学鉴定方法和生态习性分析,结合分子生物学方法分析该真菌rDNA内转录间隔区(internal transcribed spacer,ITS)序列.结果 形态学特征及生态习性表明,该大型真菌为真菌界担子菌门蘑菇纲鬼笔亚纲鬼笔目笼头菌科柄笼头菌属黄柄笼头菌(Simblum periphragmoides Klotzsch),分子生物学方法测定该真菌rDNA-ITS片段的扩增产物长度为675 bp,登录NCBI进行Blast比对,其近似物种分类单元为真菌界担子菌门蘑菇纲鬼笔亚纲鬼笔目鬼笔科鬼笔属,未具体分类到种;该野生大型真菌的ITS序列已上传GenBank,登录号为No:MT260887.结论 首次报道新疆可采集到黄柄笼头菌,为其进一步研究及利用奠定基础;野生大型真菌的分子生物学鉴定方法需要进一步优化.
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