多重聚合酶链反应系统用于儿童常见细菌及真菌血流感染的价值研究
wf（2017）
Abstract
目的：探讨多重聚合酶链反应系统用于儿童常见细菌及真菌血流感染的价值。方法选取2014年1月－2016年1月医院住院疑诊血流感染患儿135例，采集患儿的血标本，分别采用血培养法、16S rDNA‐PCR和多重聚合酶链反应系统进行检测，对比3种方法检测儿童血流感染的阳性率。结果血培养法检测阳性标本12份，阳性率为8．89％，16S rDNA‐PCR检测阳性标本26份，阳性率为19．26％，多重聚合酶链反应系统检测阳性标本24份，阳性率为17．78％；16S rDNA‐PCR和多重聚合酶链反应系统的阳性率均显著高于血培养法（P＜0．05）；16S rDNA‐PCR和多重聚合酶链反应系统阳性率比较，差异无统计学意义。结论多重聚合酶链反应系统具有操作简单、检测时间短等优势，可在较短时间内鉴定儿童血流感染的常见病原菌，对临床诊疗有较好的指导意义。
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Key words
M ultiplex polymerase-chain-reaction system,Bloodstream infection in child,Pathogen
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