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A Resource of Targeted Mutant Mouse Lines for 5,061 Genes

Nature Genetics(2021)

Université de Strasbourg | RIKEN BioResource Research Center | Wellcome Sanger Institute | Baylor College of Medicine | MRC Harwell Institute | Institute of Developmental Genetics | Monterotondo Mouse Clinic | National Laboratory Animal Center | The Jackson Laboratory | SKL of Pharmaceutical Biotechnology and Model Animal Research Center | Centre for Phenogenomics | European Molecular Biology Laboratory | German Mouse Clinic | Czech Centre for Phenogenomics | Korea Mouse Phenotyping Center (KMPC) and Graduate School of Cancer Science and Policy | Korea Mouse Phenotyping Center (KMPC) and Lee Gil Ya Cancer and Diabetes Institute | CAM-SU Genomic Resource Center | Mouse Biology Program | Immune and Vascular Cell Network Research Center | Korea Mouse Phenotyping Center (KMPC) and BK21 Program for Veterinary Science

Cited 75|Views38
Abstract
The International Mouse Phenotyping Consortium reports the generation of new mouse mutant strains for more than 5,000 genes, including 2,850 novel null, 2,987 novel conditional-ready and 4,433 novel reporter alleles.
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Experimental models of disease,Gene targeting,Genetic engineering,Mutagenesis,Stem cells,Biomedicine,general,Human Genetics,Cancer Research,Agriculture,Gene Function,Animal Genetics and Genomics
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要点】:本文报道了国际小鼠表型联盟生成超过5000个基因的新型突变小鼠品系,包括2850个新型敲除、2987个新型条件性敲除和4433个新型报告基因等位基因,为基因功能研究提供了重要资源。

方法】:通过基因编辑技术,如CRISPR/Cas9等,针对特定基因进行精确突变,生成不同的突变小鼠品系。

实验】:实验中创建了5000多个基因的突变小鼠品系,具体数据包括2850个新型敲除品系、2987个新型条件性敲除品系和4433个新型报告基因品系。数据集名称未在摘要中提及,但实验结果证实了这些品系的生成。