果子狸多态性微卫星位点的筛选及特性分析
Acta Scientiarum Naturalium Universitatis Pekinensis（2021）
Abstract
以采自四川平武县老河沟自然保护区的一份果子狸(Paguma larvata)组织样品为实验材料,通过FIASCO法构建微卫星文库,对250个单克隆进行测序分析,获得147条含微卫星序列的片段,微卫星单元重复次数大于10的序列共有42条.据此设计引物42对,进一步使用21份果子狸样品检测这些引物的扩增能力和扩增序列多态性,最终得到5个多态性微卫星位点.同时,对已发表的13个果子狸微卫星位点进行检测,发现其中5个位点具有多态性.对这10个位点的特性进行分析,结果显示它们具有较高的多态性(等位基因数为2～11,观测杂合度为0.286～0.737,期望杂合度为0.358～0.906);PID和PID-sib值表明,在约109个没有亲缘关系的个体或104个有亲缘关系的个体中,可能发现一对基因型相同的个体,由于果子狸野外局域种群规模远低于这个量级,这10个微卫星位点可用于果子狸的个体识别.
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