三种虫生菌协同毒性及与有机肥耦合效应研究
South China Agriculture（2022）
Abstract
为揭示白僵菌、绿僵菌、淡紫紫孢菌对蚜虫、地蚕、根结线虫的毒性大小,并探索生防菌与有机肥耦合效应,采用PDA对白僵菌、绿僵菌、淡紫紫孢菌对其进行活化培养,并稀释不同浓度菌剂作用于蚜虫,观察单独菌种下毒性大小,然后利用对峙法研究菌种间拮抗性,寻求最优协同杀虫组合,最后利用响应面法设计不同有机肥与菌剂配比,分析混合配方对防效的影响.结果表明:三种虫生真菌对蚜虫的毒力大小为白僵菌>淡紫紫孢菌>绿僵菌,50％致死浓度分别为5.048×106、6.962×106、1.155×107 cfu·mL-1,三种虫生菌间没有拮抗性,白僵菌与淡紫紫孢菌对蚜虫和根结线虫的协同效果最优,协同毒力指数分别为3.66、4.77,有机肥与虫生菌耦合效果影响因子大小为有机肥比例>菌剂浓度>油饼比例,并得到"有机肥80.5％+油饼14.3％+白僵菌-淡紫紫孢菌组合菌剂8.4％"配比对线虫的防效最高.
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