响应面优化亚麻荠饼粕多酚提取工艺及其抑菌和抗氧化活性研究
Abstract
以多酚得率为评价指标,单因素试验结合响应曲面分析法优化亚麻荠饼粕多酚提取工艺参数,并分别采用倍比稀释法、ABTS自由基清除能力测定法及总抗氧化性能测定法评价其体外抑菌和抗氧化活性.结果表明:在乙醇浓度45%、料液比1:45g/mL、超声温度68℃、超声时间40min的最佳提取条件下,亚麻荠饼粕多酚的得率达3.78%±0.02%,与理论预测值比较接近.亚麻荠饼粕多酚提取物对大肠杆菌、化脓性链球菌和鼠伤寒沙门氏菌具有强烈的抑制作用,MIC和MBC均在6.25mg/mL以下;亚麻荠饼粕多酚提取物具有很强的ABTS自由基清除能力及还原Fe3+的能力.优化后的亚麻荠饼粕多酚超声辅助提取工艺稳定可行,且其提取物具有较强的抑菌活性和抗氧化能力.
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