GC-C-/-小鼠在DSS诱导下肠道炎症损伤的变化
Journal of Kunming Medical University（2022）
Abstract
目的 了解鸟昔酸环化酶C(GC-C)信号通路在右旋糖醉硫酸酯钠(DSS)诱导小鼠肠道炎症性损伤发生中的作用.方法 运用DSS构建GC-C基因敲除(GC-C-/-)小鼠和野生型(WT)小鼠结肠炎模型,将小鼠分为炎症组和对照组,前者给予3％的DSS溶液自由饮用1周,后者给予正常饮食.运用qRT-PCR和Westernblot方法检测结肠组织GC-C mRNA和蛋白的表达,在光学显微镜下观察经HE染色后结肠组织的炎症损伤情况并进行组织病理学评分.运用ELISA方法检测小鼠外周血与肠黏液中炎症因子(IL-8和TNF-α)的水平.结果 (1)与WT小鼠相比,GC-C-/-小鼠在DSS作用下疾病活动指数(DAI)评分明显升高(P＜0.05),结肠缩短、肠管增粗、充血水肿更明显;(2)显微镜下观察到GC-C-/-+DSS组小鼠结肠组织炎症损伤更为严重,组织病理学评分较WT+DSS组小鼠明显升高(P＜0.05);(3)GC-C-/-小鼠在DSS诱导下外周血和肠粘液中IL-8和TNF-α水平较WT+DSS组明显升高(P＜0.05).结论 GC-C-/-小鼠在化学物质诱导下肠道炎症性损伤加重,GC-C信号通路可能参与了溃疡性结肠炎(UC)的发生与发展.
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