Microfluidic Rotating-Target Device Capable of Three-Degrees-of-freedom Motion for Efficient in Situ Serial Synchrotron Crystallography

Feng-Zhu Zhao, Zhi-Jun Wang,Qing-Jie Xiao,Li Yu,Bo Sun,Qian Hou,Liang-Liang Chen,Huan Liang,Hai Wu,Wei-Hong Guo,Jian-Hua He,Qi-Sheng Wang,Da-Chuan Yin

JOURNAL OF SYNCHROTRON RADIATION（2023）

Northwestern Polytech Univ | Chinese Acad Sci | Wuhan Univ

Cited 3|Views32

Abstract

There is an increasing demand for simple and efficient sample delivery technology to match the rapid development of serial crystallography and its wide application in analyzing the structural dynamics of biological macromolecules. Here, a microfluidic rotating-target device is presented, capable of three-degrees-of-freedom motion, including two rotational degrees of freedom and one translational degree of freedom, for sample delivery. Lysozyme crystals were used as a test model with this device to collect serial synchrotron crystallography data and the device was found to be convenient and useful. This device enables in situ diffraction from crystals in a microfluidic channel without the need for crystal harvesting. The circular motion ensures that the delivery speed can be adjusted over a wide range, showing its good compatibility with different light sources. Moreover, the three-degrees-of-freedom motion guarantees the full utilization of crystals. Hence, sample consumption is greatly reduced, and only 0.1 mg of protein is consumed in collecting a complete dataset.

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Key words

sample delivery system,serial synchrotron crystallography,microfluidic rotating-target,circular motion,in situ diffraction

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