金沙江流域捕食线虫真菌空间分布格局及其驱动机制探究
Resources and Environment in the Yangtze Basin（2023）
大理大学
Abstract
为探究金沙江流域内捕食线虫真菌(Nematode-Trapping Fungi,NTF)的空间分布格局及其驱动机制,在该流域设置了 54个采样点,分别采集各采样点的对称水体底泥、陆地土壤样品各5份.结合传统纯培养法、形态学和分子生物学方法对NTF进行分离、纯化和鉴定.共采集到样品540份,从中分离得到3属29种567株NTF,上中下游分别检出2属16种79株、2属18种205株和3属21种283株,NTF的检出率和物种丰富度从上游至下游依次递增;陆地土壤中共检出3属23种354株,水体底泥中共检出2属22种213株,陆地土壤中NTF的检出率和物种丰富度均高于水体底泥.(1)地理环境因子中,经纬度和海拔均对NTF的物种丰富度和检出率具有显著的影响(P＜0.05);(2)物候条件上,年均温对NTF物种丰富度和检出率均有显著的影响(P＜0.05),而年均降雨量对NTF的物种丰富度和检出率均影响不显著(P＞0.05);(3)在土壤理化性质方面,土壤pH、总钾和总磷对NTF物种丰富度的影响极显著(P＜0.01),土壤总磷、总钾和有机质对NTF物种检出率有显著的影响(P＜O.05),而土壤总氮对NTF的物种分布影响不显著(P＞0.05).结果表明,NTF在金沙江流域内的上中下游、水陆间存在异质性分布格局,地理因素、物候条件及土壤理化因子均在不同程度上共同驱动了这一格局的形成.
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Key words
Jinsha River,NTF,upper,middle and lower,aquatic-terrestrial link,driving factors
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