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    • Supplementary Materials and Methods, Figures 1 - 13, Table 1 from Activating and Propagating Polyclonal Gamma Delta T Cells with Broad Specificity for Malignancies

    Drew C. Deniger,Sourindra N. Maiti,Tiejuan Mi,Kirsten C. Switzer,Vijaya Ramachandran,Lenka V. Hurton,Sonny Ang,Simon Olivares,Brian A. Rabinovich,M. Helen Huls,Dean A. Lee,Robert C. Bast,Richard E. Champlin,Laurence J.N. Cooper

    openalex（2023）

    Cited 0|Views4
    Abstract
    PDF file - 1405KB, Supplementary Materials and Methods. Supplementary Figures - (1) Tumor cell line culture conditions and media formulations (2) Co-culture conditions for gamma/delta T cells and designer aAPC (3) Intracellular cytokine production, Luminex, and neutralizing antibody cytolysis assays (4) Lentivirus packaging and transduction of CAOV3 cells Contents of the Supplemental Data include figures: (1) An example of the gating strategy for gamma/delta T-cell analyses (2) Schematic of DNA plasmid pLVU3G-effLuc-T2A-mKateS158A used to co-express enhanced firefly luciferase (effLuc) and mKate (3) Expression of activation markers CD38 and CD95 on propagated gamma/delta T cells (4) aAPC developed for co-culture with gamma/delta T cells to determine the impact of introduced co-stimulatory molecules (5) Expansion of UCB-derived gamma/delta T cells on aAPC with IL-2 and IL-21 (6) Surface expression of TCRdelta1 and TCRdelta2 chains on gamma/delta T cells derived from PBMC prior to propagation (7) Abundance of Vdelta and Vgamma mRNA species in gamma/delta T cells prior to ex vivo numeric expansion (8) Surface expression of TCRdelta1 and TCRdelta2 chains on gamma/delta T cells derived from UCB and propagated on aAPC with IL-2 and IL-21 (9) mRNA expression of shared Valpha/Vdelta alleles in gamma/delta T cells separated and then propagated on aAPC, IL-2, and IL-21 (10) Abundance of mRNA species coding for Vgamma chains in gamma/delta T-cell subsets (11) In vitro lysis of tumor cell line panel by polyclonal gamma/delta T cells (12) Specific lysis of hematological and solid tumor cells by Vdelta T-cell subsets (13) Immunophenotypes associated with PBMC-derived Vdelta T-cell subsets expanded on aAPC/IL-2/IL-21. Supplemental Table 1 - details the antibodies used in this study, where they were purchased, and their hybridoma clone.
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    • Pretraining has recently greatly promoted the development of natural language processing (NLP)
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    【要点】：论文研究了通过激活和传播具有广泛肿瘤特异性的多克隆γδT细胞，以实现对恶性肿瘤的有效治疗，并探讨了使用设计型人工抗原呈递细胞(aAPC)对γδT细胞的影响。
    

    【方法】：采用共培养实验，利用设计型人工抗原呈递细胞(aAPC)激活和传播γδT细胞，并通过流式细胞术、细胞内细胞因子检测、Luminex检测以及中和抗体细胞毒性实验等方法评估γδT细胞的活性和特异性。
    

    【实验】：实验使用的数据集包括不同来源的γδT细胞（如外周血单个核细胞和脐带血来源的γδT细胞）与aAPC共培养，并在IL-2和IL-21的辅助下进行体外扩增。实验结果表明，通过这种方法可以有效地激活和传播具有广泛肿瘤特异性的γδT细胞，对多种肿瘤细胞系显示出显著的体外裂解活性。论文中的补充材料提供了详细的实验数据和图表，包括γδT细胞分析的门控策略、DNA质粒示意图、激活标记物的表达、aAPC的开发以及γδT细胞对肿瘤细胞系的裂解效果等。
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