WeChat Mini Program
Old Version Features

Expression and Localization Profiles of Tight Junction Proteins in Immune Cells Depend on Their Activation Status

Lena Voges,Franziska Weiss, Ana-Teresa Branco,Michael Fromm,Susanne M. Krug

International journal of molecular sciences(2024)SCI 2区SCI 3区

Charite Univ Med Berlin

Cited 1|Views25
Abstract
The ability of the immune system to combat pathogens relies on processes like antigen sampling by dendritic cells and macrophages migrating through endo- and epithelia or penetrating them with their dendrites. In addition, other immune cell subtypes also migrate through the epithelium after activation. For paracellular migration, interactions with tight junctions (TJs) are necessary, and previous studies reported TJ protein expression in several immune cells. Our investigation aimed to characterize, in more detail, the expression profiles of TJ proteins in different immune cells in both naïve and activated states. The mRNA expression analysis revealed distinct expression patterns for TJ proteins, with notable changes, mainly increases, upon activation. At the protein level, LSR appeared predominant, being constitutively present in naïve cell membranes, suggesting roles as a crucial interaction partner. Binding experiments suggested the presence of claudins in the membrane only after stimulation, and claudin-8 translocation to the membrane occurred after stimulation. Our findings suggest a dynamic TJ protein expression in immune cells, implicating diverse functions in response to stimulation, like interaction with TJ proteins or regulatory roles. While further analysis is needed to elucidate the precise roles of TJ proteins, our findings indicate important non-canonical functions of TJ proteins in immune response.
More
Translated text
Key words
immune cells,tight junction proteins,claudins,TAMP,angulin,LSR
求助PDF
上传PDF
Bibtex
AI Read Science
AI Summary
AI Summary is the key point extracted automatically understanding the full text of the paper, including the background, methods, results, conclusions, icons and other key content, so that you can get the outline of the paper at a glance.
Example
Background
Key content
Introduction
Methods
Results
Related work
Fund
Key content
  • Pretraining has recently greatly promoted the development of natural language processing (NLP)
  • We show that M6 outperforms the baselines in multimodal downstream tasks, and the large M6 with 10 parameters can reach a better performance
  • We propose a method called M6 that is able to process information of multiple modalities and perform both single-modal and cross-modal understanding and generation
  • The model is scaled to large model with 10 billion parameters with sophisticated deployment, and the 10 -parameter M6-large is the largest pretrained model in Chinese
  • Experimental results show that our proposed M6 outperforms the baseline in a number of downstream tasks concerning both single modality and multiple modalities We will continue the pretraining of extremely large models by increasing data to explore the limit of its performance
Upload PDF to Generate Summary
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Related Papers

Human Cathelicidin LL-37 Rapidly Disrupted Colonic Epithelial Integrity

Geeta Kilari, Jacquelyn Tran,Graham A. D. Blyth,Eduardo R. Cobo
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 2025

被引用0

Data Disclaimer
The page data are from open Internet sources, cooperative publishers and automatic analysis results through AI technology. We do not make any commitments and guarantees for the validity, accuracy, correctness, reliability, completeness and timeliness of the page data. If you have any questions, please contact us by email: report@aminer.cn
Chat Paper

要点】:研究发现免疫细胞中紧密连接蛋白的表达和定位状态依赖于细胞的激活状态,揭示了紧密连接蛋白在免疫应答中的非典型功能。

方法】:通过mRNA表达分析和蛋白质层面的绑定实验研究了不同免疫细胞在未激活和激活状态下紧密连接蛋白的表达和定位。

实验】:实验采用特定免疫细胞系,分析其在不同状态下的紧密连接蛋白表达,使用的数据集名称未在文中明确提及,但结果显示激活状态下TJ蛋白表达模式有显著变化。