Figure 4 from FUME-TCRseq Enables Sensitive and Accurate Sequencing of the T-cell Receptor from Limited Input of Degraded RNA

Ann-Marie Baker,Gayathri Nageswaran,Pablo Nenclares,Tahel Ronel,Kane Smith,Christopher Kimberley,Miangela M. Laclé,Shreerang Bhide,Kevin J. Harrington,Alan Melcher,Manuel Rodriguez-Justo,Benny Chain,Trevor A. Graham

crossref（2024）

Cited 0|Views18

Abstract

A, Hematoxylin and eosin showing the regions of FFPE adenoma and carcinoma that were macrodissected for RNA extraction and FUME-TCRseq. Scale bar, 5 mm. B, Bar chart showing the numbers of unique TCR clonotypes detected in carcinoma and adenoma regions. C, Bar chart showing the diversity of the TCR repertoire in carcinoma and adenoma regions. D, Plot showing the frequencies of the 10 most common clonotypes detected in the adenoma region and the frequencies at which they appear in the carcinoma.

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【要点】：本文介绍了一种名为FUME-TCRseq的方法，它能够从有限的降解RNA中灵敏且准确地测序T细胞受体（TCR），并在腺瘤和癌变区域中检测到独特的TCR克隆类型和TCR库多样性。

【方法】：研究使用了FUME-TCRseq技术进行TCR的测序。

【实验】：实验通过石蜡包埋组织切片（FFPE）进行宏观切割，提取RNA并进行FUME-TCRseq测序。结果在腺瘤和癌变区域中检测到了不同的TCR克隆类型，并展示了TCR库的多样性。同时，还统计了腺瘤区域中最常见的10个TCR克隆型的频率及其在癌变区域的出现频率。