Protocol for Generation of and High-Throughput Drug Testing with Patient-Derived Colorectal Cancer Organoids
STAR PROTOCOLS（2024）
Walter & Eliza Hall Inst Medial Res
Abstract
Drug sensitivity testing of patient -derived tumor organoids (PDTOs) is a promising tool for personalizing cancer treatment. Here, we present a protocol for generation of and high -throughput drug testing with PDTOs. We describe detailed steps for PDTO establishment from colorectal cancer tissues, preparation of PDTOs for high -throughput drug testing, and quantification of drug testing results using image analysis. This protocol provides a standardized workflow for PDTO testing of standard -of -care therapies, along with exploring the activity of new agents, for translational research. For complete details on the use and execution of this protocol, please refer to Tan et al. 1
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Key words
cancer,cell culture,high throughput screening,microscopy,organoids
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