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Structure-Guided Engineering of Thermodynamically Enhanced SaCas9 for Improved Gene Suppression

Eun Sung Kang,Nam Hyeong Kim,Hyun-Kyoung Lim, Hyeyeon Jeon,Kayoung Han,Young Hyun No, Kyungtae Kim,Zinah Hilal Khaleel, Dongsun Shin,Kilho Eom,Jiyoung Nam,Bok-Soo Lee, Han-Joo Kim,Minah Suh,Jaecheol Lee,Trung Thanh Thach,Jaekyung Hyun,Yong Ho Kim

Advanced Materials（2024）

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关键词
CRISPR/Cas9,CRISPR interference,domain minimization,nonviral delivery,thermostable protein engineering
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