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Development of a Duplex-ddPCR Assay for Accurate Quantification of Pseudorabies Virus Through Systematic Optimization of Amplification Bias

Zihan Tian,Hao Wu, Rong Xu,Lun Yao,Wentao Li,Qigai He

VIROLOGY(2025)

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Key words
Droplet digital PCR,Pseudorabies virus,Absolute quantification,GC-Rich template
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