Development of a LAMP Protocol to Identify the Parasitoid Carcelia Iliaca from Oak Processionary Moth (thaumetopoea Processioneae) Larval Tissue to Understand and Enhance Biocontrol Management Plans
AGRICULTURAL AND FOREST ENTOMOLOGY（2025）
Forest Res
Abstract
Oak processionary moth (Thaumetopoea processionea) (OPM) Linnaeus, 1758 (Lepidoptera: Notodontidae) is a serious forestry pest and risk to public health in the UK. The economic and environmental cost of chemical pesticides in managing OPM has driven the need for sustainable, strategies which fit into integrated pest management frameworks, including the use of novel biocontrol methods such as conservation biocontrol. Carcelia iliaca Ratzeburg, 1840 (Diptera: Tachinidae), a specific parasitoid of OPM, is currently the main biocontrol agent of the UK OPM population. However, basic information on C. iliaca life history and rates of parasitism are currently lacking, partly driven by the risks OPM pose to human health, making both study and incorporation of biocontrol into management plans difficult. Here, we design and validate a molecular diagnostic assay based on loop-mediated isothermal amplification (LAMP) to detect C. iliaca from OPM larval tissue samples collected in the field, overcoming the challenges of studying problematic invasive species such as these. To assess assay performance, diagnostic sensitivity, which was 91%, and specificity, which was 75%, are used alongside limit of detection (600 pg). We discuss the wider applications for LAMP as a cost-effective tool for studying the natural enemies of insect pests which can be used to inform conservation biocontrol management strategies.
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Key words
forest ecology,invasive species,Lepidoptera,molecular diagnostics,Tachinidae
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