Simple and Fail-safe Method to Transform Miniprep Escherichia Coli Strain K12 Plasmid DNA into Viable Agrobacterium Tumefaciens EHA105 Cells for Plant Genetic Transformation

Beenzu Siamalube, Emmanuel Ehinmitan, Maina Ngotho,Justus Onguso,Steven Runo

BIO-PROTOCOL（2025）

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Agrobacterium tumefaciens,Plasmid DNA,Freeze-thaw method,Heat-shock,Cell viability,Genetic transformation,Plant biotechnology,Escherichia coli,Potato

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