Cell-Free Synthesis System: an Accessible Platform from Biosensing to Biomanufacturing

Yongbin Chen, Wenhao Xia,Fuping Lu, Zhen Chen,Yihan Liu,Mingfeng Cao,Ning He

Microbiological Research（2025）

Cited 0|Views6

Abstract

The fundamental aspect of cell-free synthesis systems is the in vitro transcription-translation process. By artificially providing the components required for protein expression, in vitro protein production alleviates various limitations tied to in vivo production, such as oxygen supply and nutrient constraints, thus showcasing substantial potential in engineering applications. This article presents a comprehensive review of cell-free synthesis systems, with a primary focus on biosensing and biomanufacturing. In terms of biosensing, it summarizes the recognition-response mechanisms and key advantages of cell-free biosensors. Moreover, it examines the strategies for the cell-free production of intricate proteins, including membrane proteins and glycoproteins. Additionally, the integration of cell-free metabolic engineering approaches with cell-free synthesis systems in biomanufacturing is thoroughly discussed, with the expectation that biotechnology will embrace greater prosperity.

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Key words

Cell-free synthesis system,Protein synthesis,Biosensor,Biomanufacturing,Metabolic engineering

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【要点】：本文全面回顾了细胞游离合成系统，重点探讨了其在生物传感和生物制造中的应用，突出了其通过体外转录-翻译过程在生物工程应用中的巨大潜力。

【方法】：文章通过综合分析细胞游离合成系统的机制，包括生物传感器的识别-响应机制，以及细胞游离生产复杂蛋白质的策略。

【实验】：论文没有提供具体的实验细节或使用的数据集名称及结果。