A Template-Free One-Step Synthesis of Trimetallic Nano-Triangular Structures Significantly Enhances the Sensitivity of Lateral Flow Immunoassays for Acetamiprid Detection.
Analytica chimica acta（2025）
Abstract
BACKGROUND:Acetamiprid (ACE), a commonly used insecticide, is widely applied in agricultural practices to control pests. However, its potential to leave residues in crops has raised significant concerns due to the associated risks to human health through food consumption. This has made the rapid, accurate, and on-site detection of ACE residues a pressing issue in the realm of global food safety. In the present study, we developed an innovative Platinum-Copper-Nickel Alloy Nano-Triangular Structure (PCNATS) to facilitate the rapid detection of ACE using a competitive assay. The PCNATS, featuring a high specific surface area and a complex three-dimensional structure, were conjugated with anti-ACE monoclonal antibodies to create advanced nanoprobes. ELISA results demonstrated that the PCNATS significantly improved the utilization efficiency of monoclonal antibodies, leading to enhanced sensing performance. RESULTS:The PCNATS-based lateral flow immunoassay (PCNATS-LFIA) system displayed high sensitivity and accuracy, capable of quantitatively detecting ACE within 10 min. This method exhibited a limit of detection (LOD) of 3.6 ng/kg and a broad detection range from 0.05 pg/mL to 4 μg/mL. Compared to traditional gold nanoparticle-based lateral flow immunoassays (AuNPs-LFIA), the PCNATS-LFIA demonstrated a 1000-fold improvement in sensitivity. Furthermore, the assay showed strong correlation with the fitted standard curve when applied to real celery and papaya samples, achieving a satisfactory recovery rate ranging from 92.9 % to 109.9 % and 101.04 % to 115.76 %, with relative standard deviations (RSD) between 1.68 % to 7.73 % and 1.37 % to 3.02 %. SIGNIFICANCE:Therefore, the PCNATS-LFIA system offers a portable, efficient, and cost-effective solution for the rapid, on-site detection of ACE residues in agricultural products.
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