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Development and Application of a Triplex Real-Time PCR Method for the Detection of Lawsonia Intracellularis, Brachyspira Hyodysenteriae, and Clostridium Perfringens

Wenqing Wu, Lei Wang,Rui Xie, Changjiang Peng, Hao Yang, Hechao Zhu, Yajuan Luo,Lin Hua,Huanchun Chen,Bin Wu,Zhong Peng

Microbiology spectrum(2025)

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Key words
triplex real-time PCR method,Lawsonia intracellularis (LI),Brachyspira hyodysenteriae (Bhy),Clostridium perfringens (Cp),clinical detection,bacterial diarrhea diagnosis
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