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    • Biochemical Characterization, Biosynthesis Mechanism, and Functional Evaluation of Selenium-Enriched Aspergillus Oryzae A02

    Le Gao, Xiaolin Li,Yumeng Li,Zhaokun Zhang, Jialu Wang,Chao Xu,Xin Wu

    International Journal of Biological Macromolecules（2024）

    Tianjin Institute of Industrial Biotechnology

    Cited 1|Views8
    Abstract
    The synthesis mechanisms and function evaluation of selenium(Se)-enriched microorganism remain relatively unexplored. This study unveils that total Se content within A. oryzae A02 mycelium soared to an impressive 8462 mg/kg DCW, surpassing Se-enriched yeast by 2-3 times. Selenium exists in two predominant forms within A. oryzae A02: selenoproteins (SeMet 32.1 %, SeCys 14.4 %) and selenium nanoparticles (SeNPs; 53.5 %). The extensive quantitative characterization of the elemental composition, surface morphology, and size of SeNPs on A. oryzae A02 mycelium significantly differs from those reported for other microorganisms. Comparative RNA-Seq analysis revealed the upregulation of functional genes implicated in selenium transformation, activating multiple potential pathways for selenium reduction. The assimilatory and dissimilatory reductions of Se oxyanions engaged numerous parallel and interconnected pathways, manifesting a harmonious equilibrium in overall Se biotransformation in A. oryzae A02. Furthermore, selenium-enriched A. oryzae A02 was observed to primarily upregulate peroxisome activity while downregulating estrogen 2-hydroxylase activity in mice hepatocytes, suggesting its potential in fortifying antioxidant physiological functions and upholding metabolic balance.
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    Key words
    Selenium-enriched aspergillus oryzae,Selenium biotransformation,Selenoprotein
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    【要点】：本研究揭示了富硒微生物Aspergillus oryzae A02的生化特性、硒的生物合成机制以及其功能评估，发现A02菌丝体内总硒含量高达8462 mg/kg DCW，显著高于富硒酵母，并首次明确了硒在A02中的主要存在形式和生物转化机制。
    

    【方法】：通过元素定量分析、表面形态学和大小测量对A. oryzae A02菌丝体内的硒纳米粒子（SeNPs）进行详细研究，并利用比较转录组学（RNA-Seq）分析硒转化相关功能基因的上调情况。
    

    【实验】：实验使用A. oryzae A02菌种进行硒的生物富集，通过转录组测序和生物化学方法对硒的转化机制进行探究，并在小鼠肝细胞中评估了富硒A. oryzae A02对过氧化物酶体活性和雌激素2-羟基酶活性的影响。